Publication date: 25th July 2016
The actin cytoskeleton is organized by actin-binding proteins into distinct filament networks with diverse functions. Force transmission through these networks is necessary for cell movement and shape change, and applied forces can feed back on the networks to change their architecture and mechanical properties. However, measuring actin cytoskeletal forces during dynamic cellular processes in live cells has been difficult. Most measurement techniques, such as traction force microscopy, rely on transmission of forces through adhesions to an external material whose deformations can be quantified and converted into force. This talk will present a sensor of actin filament forces that permits direct measurement of cytoskeletal stresses in live cells. We use the sensor to track actin cytoskeletal stresses in cells as they undergo spreading, directed motility, and plasma membrane blebbing. Direct quantification of force transmission through the actin cytoskeleton provides a new view of dynamic cellular processes and has the potential to advance understanding of the interplay between force and function.On Thu, Jul 7, 2016 at 6:41 AM, Daniel A Fletcher <fletch@berkeley.edu> wrote:Dear Clara,My apologies for the delay. I will get the abstract in today.Best wishes, DanOn Jul 7, 2016 4:20 AM, "Cellmatrix Conference" <cellmatrix@nanoge.org> wrote:Dear Prof Fletcher, we urge you to submitt your abstract ASAP in order to be able to make the Cellmatrix Conference programme.
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