Proceedings of MATSUS Fall 2023 Conference (MATSUSFall23)
DOI: https://doi.org/10.29363/nanoge.matsus.2023.267
Publication date: 18th July 2023
Metal halide perovskite (MHP) nanocrystals (NCs) are newcomer materials with excellent features to be exploited as luminescent labels in immunochemistry and biosensing, such as high emission yields (~100%), easy synthesis, narrow emission bands, and wide color gamut. They also exhibit giant multiphoton absorption, which makes them promising NIR-label biosensing. Thus, we envision that shortly perovskite NCs will enable the manufacture of high-throughput analytical platforms for the simultaneous and sensitive detection of multiple analytes, reducing the costs of the current detection kits. However, their future relies on addressing several challenges, such as their stabilization in water, the media of serological and biological samples, the obtention of monodispersed colloidal NCs, and their size reduction. [1]
This work presents different strategies to prepare metal halide perovskite NCs stable in water and with suitable properties as luminescent tags. The approaches tackled include (A) an adaptation of the sol-gel process consisting of a post-synthetic chemical transformation of pre-synthesized NCs, either CsPbBr3 or Cs4PbX6, in the presence of silica precursors, [2-3] (B) in situ growth of perovskite NCs in silica mesoporous nanoparticles, (C) polymeric nanobeads produced by coprecipitation methods [4] and (D) adaptation of liquid atomization methods. The stabilized materials were successfully probed as fluorescent agents for staining mouse cell cultures and in immunosensing assays. Finally, we discuss open research challenges and future directions toward their final use as a luminescent tag.
References
[1] C. Collantes, W. Teixeira; et al. Appl. Mater. Today. 2023, 31, 101775
[2] C. Collantes, V. González-Pedro, et al. ACS Appl. Nano Mater. 2021, 4, 2011−2018.
[3] C. Collantes, W. Teixeira et al. J. Mater. Chem. B. Submitted.
[4] Avugadda, S. K., Castelli A. et al. ACS Nano. 2022, 16(9), 13657-13666.
This research was funded by MINECO (PID2019-110713RB-I00) and Generalitat Valenciana (PROMETEO/2020/094).
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