Publication date: 25th July 2016
By definition, living specimens are animate. Therefore, a full understanding of dynamic biological systems will only be obtained by observing them with enough 4D spatio-temporal resolution and for a sufficient duration, to capture the phenomena of interest. Unfortunately, conventional widefield or confocal microscopes are either too slow, lack the spatial resolution, or induce too much photodamage to meet these requirements. To address these limitations, we have developed Lattice Light Sheet Microscopy, a new sub-cellular light-sheet microscopy method capable of imaging fast 3D dynamic processes in vivo at signal to noise levels approaching those obtained by total internal reflection fluorescence (TIRF) illumination. By utilizing 2D optical lattices, we generate a thin (~800 nm full width half maximum) plane of light coincident with the focus of a high numerical aperture detection objective. Using this technique, we demonstrate substantial advantages in speed, sensitivity and reduced phototoxicity compared to conventional point scanning and spinning disc confocal microscopes as well as light-sheet microscopes utilizing single Gaussian or Bessel beams. We leverage these advantages to image samples ranging over three orders of magnitude in length scale from single molecules to whole embryos. Specific examples include: single molecule tracking of fluorescently tagged transcription factors in densely labeled embryonic stem cell spheroids, 3D imaging of microtubule growth phases and organelle dynamics throughout the course of cell division, 3D cell migration through collagen matrices, and protein localization throughout the course of dorsal closure in Drosophila embryos. Further, by combining lattice light sheet microscopy with point accumulation for imaging of nanoscale topography (PAINT) microscopy and novel fluorescent probes, we demonstrate 3D super-resolution localization microscopy over large fields of view and in thick 3D samples such as dividing cells and small embryos.
nanoGe is a prestigious brand of successful science conferences that are developed along the year in different areas of the world since 2009. Our worldwide conferences cover cutting-edge materials topics like perovskite solar cells, photovoltaics, optoelectronics, solar fuel conversion, surface science, catalysis and two-dimensional materials, among many others.
Previously nanoGe Spring Meeting (NSM) and nanoGe Fall Meeting (NFM), MATSUS is a multiple symposia conference focused on a broad set of topics of advanced materials preparation, their fundamental properties, and their applications, in fields such as renewable energy, photovoltaics, lighting, semiconductor quantum dots, 2-D materials synthesis, charge carriers dynamics, microscopy and spectroscopy semiconductors fundamentals, etc.
International Conference on Hybrid and Organic Photovoltaics
International Conference on Hybrid and Organic Photovoltaics (HOPV) is celebrated yearly in May. The main topics are the development, function and modeling of materials and devices for hybrid and organic solar cells. The field is now dominated by perovskite solar cells but also other hybrid technologies, as organic solar cells, quantum dot solar cells, and dye-sensitized solar cells and their integration into devices for photoelectrochemical solar fuel production.
Asia-Pacific International Conference on Perovskite, Organic Photovoltaics and Optoelectronics
The main topics of the Asia-Pacific International Conference on Perovskite, Organic Photovoltaics and Optoelectronics (IPEROP) are discussed every year in Asia-Pacific for gathering the recent advances in the fields of material preparation, modeling and fabrication of perovskite and hybrid and organic materials. Photovoltaic devices are analyzed from fundamental physics and materials properties to a broad set of applications. The conference also covers the developments of perovskite optoelectronics, including light-emitting diodes, lasers, optical devices, nanophotonics, nonlinear optical properties, colloidal nanostructures, photophysics and light-matter coupling.
The International Conference on Perovskite Thin Film Photovoltaics Perovskite Photonics and Optoelectronics (NIPHO) is the best place to hear the latest developments in perovskite solar cells as well as on recent advances in the fields of perovskite light-emitting diodes, lasers, optical devices, nanophotonics, nonlinear optical properties, colloidal nanostructures, photophysics and light-matter coupling.